Technically no — it's a dinucleotide coenzyme. We include it because reconstitution and dosing math is similar to peptide protocols.

NAD+ Calculator

NAD+ (also known as Nicotinamide Adenine Dinucleotide) reconstitution and dosage reference — calculate vial concentration, BAC water volume, and exact syringe units below.

Longevity

Key takeaways

At the reference preset of a 500mg NAD+ vial reconstituted with 5 mL of bacteriostatic water, a 100mg dose measures 100 units on a U-100 insulin syringe.

NAD+ is a coenzyme present in every living cell, central to mitochondrial energy metabolism and sirtuin activation.

Concentration: 100 mg/mL
Draw volume: 1 mL
Units (U-100): 100
Doses per vial: ≈ 5
Reference preset: 500mg · 5mL · 100mg

Reference values from the default preset — adjust the calculator below for your vial.

U-100 scale · draw to 100 units

How to use this calculator›

1. Vial — the peptide amount printed on the vial, in milligrams.
2. BAC water — the bacteriostatic water added to the vial. More water means a lower concentration and a larger, easier-to-read draw.
3. Dose — the single dose being measured, in milligrams.
4. Syringe — the barrel being drawn into. The diagram rescales to that syringe, so the same dose sits at a different mark on a 0.3 mL barrel than on a U-100.

Educational reference only — not medical advice or a dosing recommendation.

Default: 500mg / 5mL / 100mgAdjust inputs to match your vial

About

What is NAD+?

NAD+ is a coenzyme present in every living cell, central to mitochondrial energy metabolism and sirtuin activation. Subcutaneous and intravenous protocols are used in compounding-pharmacy practice for energy, recovery, and longevity-adjacent endpoints. It is not, strictly speaking, a peptide — but it is widely included in peptide-injection protocols and reconstitution math is similar.

Sourcing

Where researchers source this

Vendors below have been manually vetted for third-party COA practice and shipping reliability. Verify the current Certificate of Analysis on the vendor's product page before purchase.

Ascension Peptides

USA-based research vendor (Castle Rock, Colorado) publishing a per-batch Certificate of Analysis library with public lot IDs, so a vial can be matched to its own COA before and after purchase. Recent HPLC purity 99.79–99.98% across the catalog. Ships from the US and carries the full GLP-1 reference range alongside repair, longevity and cosmetic compounds.

Buy NAD+ 1000mg →

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Common vial sizes

100 mg	lyophilized powder
500 mg	lyophilized powder
1000 mg	lyophilized powder

Reference dose ranges

50 mg	≈ 50 U-100 units (at 500mg / 5mL)
100 mg	≈ 100 U-100 units (at 500mg / 5mL)
200 mg	≈ 200 U-100 units (at 500mg / 5mL)
500 mg	≈ 500 U-100 units (at 500mg / 5mL)

Reconstitution

How it's typically prepared

Reconstitute with BAC water; refrigerate. NAD+ subcutaneous injections can be locally painful; topical anaesthetic or split injections are sometimes used.

FAQ

Frequently asked questions

Is NAD+ a peptide?: Technically no — it's a dinucleotide coenzyme. We include it because reconstitution and dosing math is similar to peptide protocols.

Sources

References

PubMed search: NAD+ supplementation

MOTS-c — dosage calculator, reconstitution chart, and sources.
5-Amino-1MQ — dosage calculator, reconstitution chart, and sources.
Epitalon — dosage calculator, reconstitution chart, and sources.

Notice

PeptideDose is an educational reference. It is not medical advice and does not replace consultation with a licensed healthcare provider. Doses shown in presets are derived from published protocols and product labels — they are not personal recommendations.